Supplemental Material.03182014
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SUPPLEMENTAL FIGURES Supplemental Figure 1. Ex vivo erythroid differentiation of human CD34 hematopoietic progenitor cells was confirmed by flow-cytometric analysis. Dot plots show the expression of hematopoietic progenitor marker CD34, the thrombospondin receptor (CD36), transferrin receptor (CD71), glycophorin A (GPA) expression during ex vivo erythroid differentiation. Gates were defined using isotypespecific antibodies as controls. CD34 cells precipitously declined to less than 1% by day 14 (top panel) whereas cells double-positive for CD71 and GPA increased from 66% to 99% between days 4 and 14 (bottom panel). The fraction of differentiating cells expressing CD36 antigen transiently peaks between days 8 and 11 and begins to decline by day 14 (top panel).
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